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. 2012 Jun 3;1(2):163–173.

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Differentiation of GS-2 ES-cells. (A, F) GS-2 ES-cells-derived EBs. (B-E & G-J) Differentiated cell types: neuronal cells (B, G), cardiac cluster (C, H), innervated myotubes (D, I) and endoderm-like cells (E, J). A-E, phase contrast and F-J, respective fluorescence images. Magnification bars. A, F: 50 μm; B, G: 25 μm; C-E & H-J: 10 μm. (II) H-E stained teratoma sections showing ectodermally-derived epidermis (A), mesodermally-derived skeletal muscle (B) and endodermally-derived respiratory ciliated epithelium (C). Magnification bars. A-C: 25 μm.