Table 1.

Primers used for RT-PCR analysis^*

Sl. No.	Gene name	Forward and reverse primer sequences (5’→3’)	Amplicon size (bp)
1.	Oct-4	TCT TTC CAC CAG GCC CCC GGC TC	224
		TGC GGG CGG ACA TGG GGA GAT CC
2.	Nanog	AGG GTC TGC TAC TGA GAT GCT CTG	364
		CAA CCA CTG GTT TTT CTG CCA CCG
3.	Sox2	TAG AGC TAG ACT CCG GGC GAT GA	297
		TTG CCT TAA ACA AGA CCA CGA AA
4.	Rex1	AGC AGG ATC GCC TCA CTG	189
		GGC CAC TTG TCT TTG CCG
5.	Utf 1	GGA TGT CCC GGT GAC TAC GTC TG	344
		GGC GGA TCT GGT TAT CGA AGG GT
6.	Esg1	GAA GTC TGG TTC CTT GGC AGG ATG	376
		ACT CGA TAC ACT GGC CTA GC
7.	EGFP	CAC ATG AAG CAG CAC GAC TT	267
		GAA GTT CAC CTT GAT GCC GT
8.	β-Actin	TGA ACC CTA AGG CCA ACC GTG	396
		GCT CAT AGC TCT TCT CCA GGG
9.	Nestin	CCA AAG AGG TGT CCG ATC AT	213
		TGA CAT CCT GGA CCT TGA CA
10.	BMP-4	CCT GGT AAC CGA ATG CTG AT	260
		AGC CGG TAA AGA TCC CTC AT
11.	α-Feto protein	TCC TCC TGC TAC ATT TCG CT	199
		TTC TTC ATT GCA GCC AAC AC
12.	NKx2.5	CAG TGG AGC TGG ACA AAG CC	217
		TAG CGA CGG TTC TGG AAC CA
13.	GATA-4	GCA GCA GCA GTG AAG AGA TG	186
		GCG ATG TCT GAG TGA CAG GA
14.	α-MHC	CTG CTG GAG AGG TTA TTC CTC G	302
		GGA AGA GTG AGC GGC GCA TCA AGG

^*Primers sets used are from published data and are exon-spanning ones.