Table 2.
Summary on derivation efficiencies of ES-like cells from FVB/N wild-type and EGFP-transgenic blastocysts or from FVB/N GU-3X129/SvJ F1 hybrid blastocysts.
| Steps in ES-cells derivation | Values | ||
|---|---|---|---|
|
| |||
| Wild Type | Transgenic | GU3X129/SvJ | |
| No. of embryo donors* | 14 | 14 | 05 |
| No. of blastocysts recovered (Avg./donor) | 185 (13) | 48 (12) | 19 (3.8) |
| No. of blastocysts cultured (% of recovered) | 159 (85.9%) | 39 (81.3%) | 16 (84.2%) |
| No. of blastocysts hatched (% of cultured) | 140 (88.1%) | 34 (87.2%) | 16 (100%) |
| No. of blastocyst attached (% of hatched) | 128 (91.4%) | 27 (79.5%) | 16 (100%) |
| No. of ICM picked, trypsinized and plated (% of attached) | 119 (92.9%) | 24 (88.9%) | 13 (81.3% ) |
| No. of primary colonies (% of ICM picked) | 102 (85.7%) | 16 (66.7%) | 9 (69.2% ) |
| No. of colonies, past 3-8 passages (% of primary colonies) | 18 (17.6%) | 4 (25.0%) | 5 (55.6% ) |
*
Wild-type and transgenic FVB/N mice were super-ovulated with PMSG/hCG, while 129/SvJ female (mated with FVB/N-GU3 male) donors were un-stimulated.