Table 3.
Expansion Ratio | Input cells | Cell counts* (48 hrs.) | No. of colonies/dish |
---|---|---|---|
1:4 | 0.6 X 106 | 2.97 X 106 | ~42,268 |
1:8 | 0.3 X 106 | 2.04 X 106 | ~39785 |
1:16 | 0.15 X106 | 1.67 X 106 | ~28,382 |
Cell counts shown are approximate numbers based on visual countings of alkaline phosphatase stained cells from the grabbed images of the colonies.