Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

. 2013 Mar 12;(73):50145. doi: 10.3791/50145

Solution	Contents (in mM, if not specified differently)
1	117 NaCl, 4 KCl, 10 HEPES, 1 KH₂PO₄, 4 NaHCO₃, 1.7 MgCl₂, 10 Glucose
2	117 NaCl, 4 KCl, 10 HEPES, 1 KH₂PO₄, 4 NaHCO₃, 1.7 MgCl₂, 10 Glucose + 229.5 μM EGTA
3	117 NaCl, 4 KCl, 10 HEPES, 1 KH₂PO₄, 4 NaHCO₃, 1.7 MgCl₂, 10 Glucose, CaCl₂ 0.1 + 0.8 g/L Collagenase Type 2 (300 U/L)*
4	117 NaCl, 4 KCl, 10 HEPES, 1 KH₂PO₄, 4 NaHCO₃, 1.7 MgCl₂, 10 Glucose, CaCl₂ 0.2 + 0.8 g/L Collagenase Type 2 (300 U/l)* + BSA (1 g/L)
5	117 NaCl, 4 KCl, 10 HEPES, 1 KH₂PO₄, 4 NaHCO₃, 1.7 MgCl₂, 10 Glucose, CaCl₂ 0.5 + BSA (1 g/L)
6	117 NaCl, 4 KCl, 10 HEPES, 1 KH₂PO₄, 4 NaHCO₃, 1.7 MgCl₂, 10 Glucose, CaCl₂ 1.0 + BSA (1 g/L)