Figure 8.

Fluconazole inhibits the forskolin (FSK)-induced redistribution of AQP2 in primary IMCD cells. (A) Primary IMCD cells were left untreated, stimulated with forskolin (10 µM, 20 minutes), or treated with fluconazole (10 µM, 30 minutes) alone or in combination with forskolin. AQP2 was detected by immunofluorescence microscopy using specific primary antibodies (H27) and Cy3-coupled antirabbit secondary antibodies (red).⁵¹ Nuclei were stained with DAPI (blue). Shown are representative images from one of three independent experiments. (B) The intensities of intracellular and plasma membrane immunofluorescence signals arising from AQP2 were determined and related to nuclear signal intensities (n≥30 cells per condition; mean ±SEM; three independent experiments). The ratios of plasma membrane to intracellular fluorescence signal intensities were calculated. Ratios >1 indicate a predominant localization at the plasma membrane. ***P<0.001.