FIGURE 3.

A, indicated cell lines were infected with recombinant adenoviruses expressing β-galactosidase (B) or NEUROG3 (N). Total RNA was extracted 48 h after infection and expression of the endogenous Neurog3 mRNA and the NEUROG3 transgene was determined by RT-PCR. Note that the endogenous Neurog3 gene was only activated in ductal mPAC (mouse) and PANC-1 (human) cells despite the fact that the NEUROG3 transgene was present in all studied cell lines. The housekeeping Actb gene was used as loading control. Images are representative of a minimum of two independent experiments. B, immunocytochemistry using an antibody specific for mouse Neurog3 in mPAC and MIN6 cells 48 h after treatment with the indicated adenoviruses. C, RT-PCR showing expression of endogenous mRNAs encoding pancreatic progenitor transcription factors in the indicated mouse cell lines. Total RNA from E14.5 embryonic pancreas was included as a positive control for all assayed genes.