FIGURE 6.

A and B, −4.9 kb mouse Neurog3 promoter luciferase reporter vector or the backbone luciferase vector were cotransfected with a CMV promoter-driven Renilla luciferase reporter plasmid and CMV-driven expression vectors encoding Foxa1, Neurog3, E47, or an empty vector into mPAC (A) and NIH3T3 (B) cells as indicated. Reporter gene activity is expressed relative to the activity of the promoter-less parental vector pFOXLuc and the empty expression vector, set at 1. All data are presented as the mean ± S.E. from 3–4 independent experiments. *, p < 0.05 versus no cDNA (empty bar), #, p < 0.05 versus Neurog3+E47. C, endogenous Foxa1 and Foxa2 mRNA expression in the indicated mouse cell lines was assessed by RT-PCR. Actb is included as loading control. Image is representative of two independent experiments. D, total RNA from mPAC cells was collected 44–46 h after infection with recombinant adenoviruses expressing B-gal or NEUROG3. Foxa1 and Foxa2 mRNAs were quantified relative to the Tbp gene by real time RT-PCR and expressed as fold-increase versus expression in cells treated with B-gal set at 1. Results are mean ± S.E. from four independent experiments. p < 0.01 versus B-gal.