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. 2013 Feb 25;288(17):11718–11730. doi: 10.1074/jbc.M113.452276

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Recombinant SiaD_W-135 synthesizes high molecular weight W-135 polymer chains from short oligosaccharide primers. A, to test if recombinant SiaD_W-135 can elongate short oligomer primers, CPS isolated from Nm_W-135 was hydrolyzed (CPS(h)) and used to prime the polymerase reaction. The production of long polymer chains demonstrated an active enzyme. High percentage PAGE and Alcian blue/silver staining was used to display starting, as well as synthesis products. B, CPS(h) produced from CPS was used in a time course experiment. Here products were analyzed on a 10% SDS-PAGE to additionally visualize the enzyme.