FIGURE 8.
CMP-Neu5Ac and UDP-Gal bind independently to SiaDW-135. To find out if binding of one nucleotide sugar impacts binding of the second, the STD NMR spectrum of CMP-Neu5Ac in complex with SiaDW-135 was recorded in the absence (a) and presence (b) of an equimolar concentration of UDP-Gal. No change in the position or intensity of any signal was seen, thus indicating independent binding of the nucleotide sugars. To highlight this fact, an overlay is shown for the intense signal produced by the tightly bound N-acetamido group (c). For experimental details, see Fig. 6.