Primer pair used for cloning |
KS422/KS273 |
5′-GCATCTCATATGGCTGTTATTATATTTGTTAACG-3′ |
|
5′-CCGCTCGAGTTTTTCTTGGCCAAAAAACTG-3′ |
|
Primer pairs used for the introduction of mutations |
|
KS350/KS351 |
5′-CTGATCATGACATCAGAAAGTGCGGGATTTCCATATATATTTATG-3′ |
|
5′-CATAAATATATATGGAAATCCCGCACTTTCTGATGTCATGATCAG-3′ |
KS364/KS365 |
5′-CAACTACAGCTTTTATTTTCTAAGGCTCCAGATGAAAATATAGATTTAAAG-3′ |
|
5′-CTTTAAATCTATATTTTCATCTGGAGCCTTAGAAAATAAAAGCTGTAGTTG-3′ |
KS366/KS367 |
5′-CTACAGCTTTTATTTTCTAAGCATGCAGATGAAAATATAGATTTAAAGAAC-3′ |
|
5′-GTTCTTTAAATCTATATTTTCATCTGCATGCTTAGAAAATAAAAGCTGTAG-3′ |
KS370/KS371 |
5′-ATCTCGCGTTGCTGTAGGTGTTTATGCAACTAGCTTATTTG-3′ |
|
5′-CAAATAAGCTAGTTGCATAAACACCTACAGCAACGCGAGAT-3′ |
KS372/KS373 |
5′-CGTTGCTGTAGGTGTTTATTCAGCTAGCTTATTTGAGGCATTAG-3′ |
|
5′-CTAATGCCTCAAATAAGCTAGCTGAATAAACACCTACAGCAACG-3′ |