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Minimal backbone chemical shift (15N, 13C′, and 1HN) change values observed for hPD-1 on hPD-L1 (A) or hPD-L2 (B) binding. The values were obtained by comparison of three-dimensional TROSY-HNCO spectra of the samples consisting of the free or hPD-L1/hPD-L2-bound 2H/13C/15N-labeled hPD-1.
