TABLE 2.
Enzyme | Km | kcat | kcat/Km |
---|---|---|---|
×1−6 m | s−1 | ×103 s−1/m | |
Wild type | 99 ± 11 | 15 ± 1 | 160 ± 10 |
H263F | 120 ± 20 | 0.15 ± 0.01 | 1.3 ± 0.1 |
D320A | 130 ± 20 | 0.0068 ± 0.0005 | 0.052 ± 0.006 |
D320N | 380 ± 50 | 0.011 ± 0.001 | 0.028 ± 0.002 |
Y419F | –a | 0.086b | – |
a The Km value was too low to be determined.
b The v/[E]0 value at 100 μm substrate.