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. 2013 Mar 18;288(17):11824–11833. doi: 10.1074/jbc.M112.432302

FIGURE 3.

FIGURE 3.

OPN influences drug resistance through the regulation MDR genes, ABCB1 and ABCG2. A–F, exogenous OPN induced resistance to doxorubicin (A and B), paclitaxel (C and D), and cisplatin (E and F) in MCF7 (*, p = 0.01; p = 0.0002; p = 0.02) and SUM159 (*, p > 0.05; p = 0.01; p = 0.01); cells, respectively. The response to the indicated drug concentrations was assessed by MTS assay. G, blocking the transcriptional activity of GLI using GANT61 significantly decreased expression of ABCB1 and ABCG2 in MCF7 (*, p < 0.0001 for both ABC proteins) and SUM159 (*, p = 0.01 and p = 0.002, respectively) cells (H). OPN caused a notable increase in ABCB1 and ABCG2 in MCF7 (†, p < 0.0001 for both ABC proteins) and SUM159 (†, p = 0.0002 and p = 0.0009, respectively) cells. GANT61 negated the effect of OPN on the expression of both MDR genes (*, p < 0.0001), ABCB1 and ABCG2. MCF7 cells or SUM159 cells were treated with either GANT61 (10 μm) or OPN (100 ng/ml) or OPN and GANT61. RNA was assessed for the expression of ABCB1 and ABCG2 by qRT-PCR. Error bars, S.E.