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. 2013 Mar 15;288(17):11854–11864. doi: 10.1074/jbc.M112.441915

FIGURE 3.

FIGURE 3.

Growth inhibition resulting from expression of DrrB alone or DrrAB together in E. coli 797 cells can be relieved by overexpression of FtsH or GroESL. A, effect of overexpression of FtsH or GroESL on growth of 797 cells expressing DrrB. 797 cells containing pDX103 (DrrB) and pUCftsH or pUCgroESL were grown at 30 °C and induced at 42 °C, as described in the legend to Fig. 1B. The growth was monitored at A600 nm for 4 h after induction. B, effect of overexpression of FtsH or GroESL on growth of 797 cells expressing DrrAB. 797 cells containing pDX101 (DrrAB) and pUCftsH or pUCgroESL were anayzed, as described in A above. C, quantitation of the growth of 796 or 797 cells expressing DrrB or DrrAB at 4 h after induction. The growth of sample #1 at 4 h in A or B was designated as 1.0. The relative growth of each culture at 4 h was calculated. The histogram represents an average of 3 experiments. Columns 1–9 correspond to cultures 1–9 in A (filled) and B (open). D, Western blot analysis. The membrane fractions were prepared from samples 6–9 collected at the 4-h time point in A and B. Western blot analysis was carried out as described in the legend to Fig. 1A.