FIGURE 7.

The AAA domain of FtsH is sufficient to complement the growth defect resulting from the expression of DrrAB in 797 cells. E. coli 797 cells containing the drrAB genes (pDX101) and the wild-type or a mutated version (K198N, HEH, or AAA subclone) of the ftsH gene on a compatible plasmid were grown at 30 °C and induced at 42 °C, as in Fig. 1B. A, growth analysis at 600 nm. B, Western blot analysis. Membranes prepared from the cells collected at the 3-h time point were analyzed, as described in the legend to Fig. 1A. C, quantitative analysis of DrrA and DrrB. The intensity of the bands on the nitrocellulose membrane in B was determined by densitometric scanning. The intensity of DrrA and DrrB in lane 1 was designated as 1.0. The data represent an average of 3 experiments. Columns 1–8 correspond to samples 1–8 in B.