TABLE 1.
Bacterial strains, plasmids, and antisera
| Name | Description | Ref. or source |
|---|---|---|
| Bacterial strains | ||
| TG1 | K-12Δ (lac-pro) supE thi hsdD5/F′ tra36 proA+B+ lacIq lacZΔM15 | 28 |
| HMS174(DE3) | F− recA1 hsdR (rK12- mK12+) (DE3) (Rif R) | 28 |
| AR796 | F−, zhd-33::Tn10, araD139, (argF-lac)U169, rpsL150, rpsE, relA1, f1bB5301, deoC1, ptsF25, rbsR, zgj3198::Tn10kan. | 29 |
| AR797 | F−, zhd-33::Tn10, araD139, (argF-lac)U169, rpsL150, rpsE, relA1, f1bB5301, deoC1, ptsF25, rbsR, zgj3198::Tn10kan. ftsH1 | 29 |
| SG1110 | Δlon-510, zba-1091::ΔTn10 (λ) | 30 |
| SG1126 | Δarg, clpA319::Δkan | 30 |
| Plasmids | ||
| pSU2718 | Cloning vector, pACYC184 derivative, Cmr | 1 |
| pKY326 | groES/groEL in pHSG575, Cmr | 19 |
| pDX101 | drrAB in pSU2718, Cmr | 1 |
| pDX103 | drrB in pSU2718, Cmr | 1 |
| pUCftsH | ftsH in pUC18, Ampr, restriction sites: EcoRI, HindIII | This study |
| pUCftsH(K198N) | pUC18 containing ftsH with mutation of Lys198 to Asn, Ampr | This study |
| pUCftsH(HEH) | pUC18 containing ftsH with mutations of His417-Glu418-His421 to Ala417-Gln418-Ala421, Ampr | This study |
| pUCftsH(AAA) | pUC18 containing ftsH with deletion of proteolytic domain, Ampr | This study |
| pUCgroESL | groES/groEL in pUC18, Ampr, restriction sites: NdeI, HindIII | This study |
| pETftsH | ftsH in pET28a with C-terminal histag, Kanr | This study |
| pETFftsH(K198N) | ftsH(K198N) in pET28a with C-terminal His tag, Kanr | This study |
| pETftsH(HEH) | ftsH(HEH) in pET28a with C-terminal His tag, Kanr | This study |
| pETftsH(AAA) | ftsH(AAA) in pET28a with C-terminal His tag, Kanr | This study |
| pBADftsH | ftsH in pBAD/HisA, Ampr | This study |