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. 2013 Mar 13;288(17):11877–11886. doi: 10.1074/jbc.M112.442491

FIGURE 4.

FIGURE 4.

Effects of sLR11 on HSPC adhesion to bone marrow stromal cells. A, U937 cells preincubated for 2 h with the indicated concentrations of sLR11 were subjected to analyses of their adhesion to plates coated with MSCs as described under “Experimental Procedures.” B, cells transiently transfected with cDNA specific for LR11 or with an empty vector (mock) were subjected to adhesion analyses as described for A. Inset, LR11 expression of mock-transfected and LR11-overexpressing U937 cells was analyzed by immunoblotting. C, c-Kit+ Lin− cells preincubated for 15 min with the indicated concentrations of sLR11 were subjected to analyses of their adhesion to plates coated with OP-9 cells as described under “Experimental Procedures.” In all panels, the numbers of attached cells are presented as -fold increase of those under control conditions (mean ± S.D. (error bars), n = 3). *, p < 0.05; ns, not significant.