TABLE 3.

Kinetic parameters of wild-type MCD and the C206S/C243S, C206S, C243S, and E302G variants under non-oxidative and oxidative conditions

Oxidant (H2O2) concentration	Variant	MCD kinetic parameters
		kcata	S0.5 (malonyl-CoA)	n (Hill coefficient)	kcat/S0.5
		s−1	mm		m−1 s−1
0 m	WT	141.2 ± 2.1	0.83 ± 0.03	1.04 ± 0.02	0.17
0.05 m	WT	128.3 ± 4.6	0.46 ± 0.04	1.14 ± 0.08	0.28
0.1 m	WT	135.0 ± 4.2	0.42 ± 0.03	1.28 ± 0.09	0.32
0.2 m	WT	109.2 ± 3.8	0.35 ± 0.03	1.43 ± 0.12	0.31
0 m	C206S/C243S	162.5 ± 7.5	1.16 ± 0.13	1.04 ± 0.06	0.14
0.1 m	C206S/C243S	175.4 ± 9.2	0.99 ± 0.13	1.06 ± 0.07	0.18
0.2 m	C206S/C243S	167.1 ± 7.1	0.81 ± 0.09	0.98 ± 0.05	0.20
0 m	C206S	117.1 ± 1.7	0.73 ± 0.02	1.17 ± 0.02	0.16
0.1 m	C206S	141.2 ± 8.8	1.04 ± 0.16	1.11 ± 0.09	0.14
0.2 m	C206S	114.2 ± 4.2	0.79 ± 0.08	1.04 ± 0.06	0.15
0 m	C243S	94.6 ± 2.1	0.58 ± 0.03	1.19 ± 0.05	0.16
0.1 m	C243S	137.5 ± 5.4	0.68 ± 0.07	1.25 ± 0.09	0.20
0.2 m	C243S	208.3 ± 2.5	0.56 ± 0.02	1.37 ± 0.04	0.37
0 m	E302G	13.3 ± 8.8	0.22 ± 0.13	0.43 ± 0.07	0.06

^a k_cat was computed for monomers.