FIGURE 5.

Perturbations caused by the binding of ZnPPIX or heme. A and B, residues (indicated by the black dots) affected by ZnPPIX reconstitution. Perturbed residues clustered around strand Aβ, helix Cα, and the D-loop are shown in panel A. Perturbed residues clustered around the dimeric interface between the HI-loop and the A′α domain are shown in panel B. C, partial ¹H-¹⁵N HSQC spectra before (left panels) and after (right panels) ZnPPIX reconstitution. D, residues affected in the WEFT-HSQC experiment upon heme reconstitution. A total of 12 residues (indicated by the black dots) are affected upon the reconstitution with heme. The residues are within the vicinity of helix Cα, the D-loop, the GH-loop, the HI-loop, and the A′α domain. E, ¹H-¹⁵N WEFT HSQC spectra before heme reconstitution (left panel) and after heme reconstitution (right panel) are shown.