FIGURE 3.

med16Δ diminishes recruitment of the Tail subunit Med3 to induced HSP promoters while obviating recruitment of subunits representative of the other three modules. A, occupancy of TAP-tagged Head, Middle, Tail and Kinase subunits within the SSA4 promoter in isogenic MED⁺ and med16Δ cells in a heat shock time course. TAP ChIP analysis was conducted as described in the legend to Fig. 1. Occupancy of each subunit in the MED⁺ strain at T = 0 min was assigned a value of 1; all other values (MED⁺ and med16Δ) are normalized to it. Only ChIP values above the beads alone background are shown. Depicted are means ± S.D. (error bars); n = 3. B–D, as in A, except Mediator subunit occupancy at the HSP104, HSP82, and ZPR1 promoters is depicted.