TABLE 1.
Yeast strains
| Strain | Genotype | Source/Reference |
|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Research Genetics |
| med16Δ | BY4741; med16Δ::KANMX | Research Genetics |
| med15Δ | BY4741; med15Δ::KANMX | Research Genetics |
| cdk8Δ | BY4741; cdk8Δ::KANMX | Research Genetics |
| SYK1006 | BY4741; Med18-TAP::HIS3MX10 | This study |
| SYK1007 | BY4741; Med4-TAP::HIS3MX10 | This study |
| SYK1008 | BY4741; Med3-TAP::HIS3MX10 | This study |
| SYK1009 | BY4741; CycC-TAP::HIS3MX10 | This study |
| SYK1010 | med16Δ; Med18-TAP::HIS3MX10 | This study |
| SYK1011 | med16Δ; Med4-TAP::HIS3MX10 | This study |
| SYK1012 | med16Δ; Med3-TAP::HIS3MX10 | This study |
| SYK1013 | med16Δ; CycC-TAP::HIS3MX10 | This study |
| SYK1014 | med15Δ; Med3-TAP::HIS3MX10 | This study |
| SYK1015 | BY4741; med15Δ::KANMX med16Δ::KANMX Med3-TAP::HIS3MX10 | This study |
| SYK1000 | BY4741; med19-1001 | This study |
| S7JJ2a | BY4741; med21-1002 | Jenny Jeon |
| N2JJ1a | BY4741; med10-1001 | Jenny Jeon |
| W303–1A | MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 | Rodney Rothstein |
| SYK1016 | W303-1A; hsf1Δ::KANMX Med4-TAP::HIS3MX10 pEVS103 (HSF1+ TRP1 CEN ARS) | This study |
| SYK1017 | SYK1016; MATα pHF312 (HSF1ΔCTA) | This study |
| SYK1018 | SYK1017; pHF35 (HSF1ΔNTA) | This study |
| SYK1019 | SYK1017; pHF71B (HSF1ΔNTA ΔCTA) | This study |
| DY150 | MATa ade2-1 ura3-1 his3-11,15 trp1–1 leu2–3,112 can1–100 | Ref. 45 |
| DY2694 | DY150; rgr1-Δ2::LEU2 | Ref. 45 |
| SLY101 | MATα ade− can1-100 cyh2r his3-11,15 leu2-3,112 trp1-1 ura3 | Ref. 76 |
| HS1004 | SLY101; TRP1+ hsp82-ΔHSE1 hsp82-ΔHSE1/HIS3::URA3 hsp82-ΔHSE1/lacZ::leu2 | Ref. 20 |