TABLE 1.

qRT-PCR and ChIP primer sequences of listed genes with amplicon length

Gene name (human)	Sequence (5′ → 3′)	Size
qRT-PCR primersa
GAPDH
Sense	AAGGTCGGAGTCAACGGATTTGGT	534
Antisense	AGTGATGGCATGGACTGTGGTCAT
SPDEF
Sense	CAGGTGAAGTCCGCTCTTTC	205
Antisense	AATGTGCAGAAGTGGCTCCT
E-cadherin
Sense	CGAGAGCTACACGTTCACGG	119
Antisense	GGGTGTCGAGGGAAAAATAGG
ChIP primers
E-cadherin
Sense	CTCACCTGGCTGCAGCCACGC	294
Antisense	GCGGTGACGACGGGAGAGGA
Control
Sense	TGGTGGGGAGAGTTGTGGCT	115
Antisense	TCGGTGACTCAGGGCCCCAC

^a Melting curve analysis was performed to assure that only one PCR product was formed. Primers were designed to generate a PCR amplification product of 100–550 bp. Only primer pairs yielding unique amplification products without primer-dimer formation were subsequently used for real time PCR assays.