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. 2013 Mar 12;288(17):12270–12282. doi: 10.1074/jbc.M112.411884

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — DEFB114 inhibits LPS-mediated MAPK phosphorylation in macrophages (RAW264.7). Macrophages (RAW264.7) were incubated with DEFB114 (or alkylated DEFB114) and then LPS, or left untreated. Cellular proteins were extracted and separated by SDS-PAGE and blotted onto PVDF membranes. LPS signaling via MAPK p42/44 (a) was detected using a phospho-specific antibody. a, decreased intensity of phospho-specific bands (p-MAP kinase p42/44) accompanied with increasing DEFB114 concentrations in comparison with total MAPK p42/44 and actin. Alkylated DEFB114 failed to inhibit the phosphorylation of MAPK p42/44 in comparison with its intact form (b).