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Structure determination of RiAFP. A, preliminary electron density map generated by Arp/wArp from initial phases. The 2Fo − 2Fc map is in blue and contoured at 1.3σ, whereas the Fo − Fc map is in green and red (indicating positive and negative peaks, respectively) and contoured at +3.0σ. B, model used in P31 molecular replacement. Despite building most of the final model, no connectivity was observed between individual β-strands in the higher (P3121) space group.
