Figure 3.

Unique altered expression signatures characterize the TGF-β pathway profiles of BM and spleen of Gata1^low mice. (A) Hierarchical clustering of normalized gene expression in BM and spleen from untreated wild-type and Gata1^low mice and from Gata1^low treated with either vehicle or SB431542, as indicated (each line a different mouse, 3 mice per experimental group). (B) Venn diagram depicting overlap of differentially expressed genes in BM and spleen of untreated Gata1^low mice or in Gata1^low mice treated with either vehicle or SB431542, as indicated. Only genes with ≥ 2-fold differences in expression levels were considered. For further details, see Table 3. (C) Quantitative RT-PCR determinations of the mRNA levels for Acvrl1, Bmp2, and Igf1 in BM and of Id2, Stat1, and Ltbp1 in spleen of untreated wild-type and Gata1^low mice and of Gata1^low mice treated with either vehicle or SB431542, as indicated. Results are normalized to those observed in wild-type mice and are presented as mean (±SD) of those observed, with 3 mice per experimental group. Values statistically different (P < .05) from those observed in wild-type or untreated Gata1^low mice are indicated with * or ^&. Differences in gene expression among the 4 experimental groups are similar to those observed by microarray analyses.