Table 3.
Comparison of the cytokine content of BM and spleen washes from 10-mo-old wild-type and Gata1low mice
| BM | Spleen | |
|---|---|---|
| IFNγ | 72 ± 15 | 68 ± 25 |
| IL- 2 | 67 ± 1 | 154 ± 36 |
| IL- 3 | 80 ± 1 | 82 ± 15 |
| IL- 5 | 85 ± 6 | 37 ± 12 |
| IL- 9 | 74 ± 8 | 88 ± 5 |
| IL- 12 | 109 ± 5 | 81 ± 2 |
| CXCL-1 | 65 ± 11 | 76 ± 7 |
| RANTES | 219 ± 49 | 98 ± 25 |
| TNFα | 75 ± 11 | 62 ± 11 |
The content of 19 cytokines [GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-13, IL-17, CXCL1, MCP-1, M-CSF, RANTES, and TNFα] was determined. The concentrations of GM-CSF, IL-1α, IL-1β, IL-4, IL-6, IL-10, IL-13, IL-17, and M-CSF were below detection (<1 pg/50 μg total protein) in all the samples analyzed. The concentrations of the other cytokines were determined using 3 mice per experimental group and are presented as mean (±SD) percent of the total cytokine content per organ detected in wild-type animals. Bold fonts indicate Gata1low values significantly (P < .05) lower than those observed in wild-type mice