Table 6.
Frequency of progenitor cells, erythroid cells, and MK precursors in the BM and spleen of vehicle- and SB431542-treated Gata1low mice
| Wild type | Gata1low |
|||
|---|---|---|---|---|
| Untreated | Vehicle treated | SB431542 treated | ||
| CD117pos | ||||
| BM | 9.8 ± 3.5 | 3.5 ± 1.2* | 6.5 ± 1.9 | 7.8 ± 1.1† |
| Spleen | 0.2 ± 0.5 | 4.6 ± 2.1* | 6.6 ± 2.1 | 11.5 ± 2.1† |
| CD71pos/Ter119pos, % | ||||
| BM | 19.3 ± 8.1 | 13.9 ± 4.3 | 14.7 ± 1.6 | 26.7 ± 2.8† |
| Spleen | 8.5 ± 2.9 | 14.2 ± 5.5* | 12.7 ± 3.6 | 13.2 ± 2.3 |
| CD41pos/CD61neg, % | ||||
| BM | 9.9 ± 5.8 | 30.5 ± 8.7* | 30.9 ± 3.1 | 28.8 ± 4.6 |
| Spleen | 12.8 ± 5.7 | 23.7 ± 3.1* | b.d.‡ | b.d.‡ |
| CD41pos/CD61pos, % | ||||
| BM | 3.8 ± 1.0 | 8.1 ± 2.5* | 14.5 ± 4.7 | 10.6 ± 3.4 |
| Spleen | 2.3 ± 1.1 | 19.4 ± 2.3* | 16.7 ± 3.4 | 10.7 ± 3.0‡ |
Data are presented as mean (±SD) of 3-6 determinations per experimental point. MK precursors were defined as CD41pos cells and divided into immature (CD61neg) and mature (CD61pos) MKs. *, †, and ‡, P < 0.05 between untreated Gata1low and wild-type mice, SB431542- and vehicle-treated Gata1low mice, or treated and untreated Gata1low mice, respectively. The cloning efficiency of CD117pos cells prospectively isolated from vehicle- and SB431542-treated animals was 55 ± 12 vs 44 ± 5 CFC/103 plated cells, respectively.
b.d., below detection.