Fig. 5. Knd1p is required for efficient CRL1-mediated substrate degradation.

(a) Wildtype and Δknd1 cells were incubated with 100 ug/ml cycloheximide for the indicated times and Ams2p levels were determined by immunoblotting relative to the loading control Cdc2p. The graph on the right shows a quantification of the results. Exponential trend lines were fitted through the data points in Excel, and the resulting equations were used to calculate half-lives.

(b) Hydroxyurea sensitivity of Δknd1 cells. 5-fold serial dilutions of wildtype and Δknd1 cells were spotted onto media with (bottom) or without (top) 10 mM hydroxyurea (HU).