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. Author manuscript; available in PMC: 2013 Apr 26.
Published in final edited form as: Protein Expr Purif. 2011 Oct 19;81(2):175–180. doi: 10.1016/j.pep.2011.10.006

Figure 5. FT-ICR MS spectrum of the products of GalNAc-T2 reaction after overnight incubation.

Figure 5

Hinge-region peptide was used as acceptor with 1 protein, corresponding to 1.85 Units of enzyme. The reaction products were purified from the mixture by LC coupled on-line with LTQ FT-ICR MS. Predominant ion species corresponded to hinge-region glycopeptides with 6, 7, and 8 GalNAc residues. Minor species with 5 and 9 residues are shown in the insets. The number next to square symbol indicates number of GalNAc residues attached. All glycopeptides were detected at LC retention time of 31.5–34.0 min. All glycopeptides were detected as 3+ charged ions. *adducts with NH3 and matched the theoretical mass values for the expected hinge-region glycopeptides within 2 ppm or less.