Figure 5. Antiviral activity is associated with specific glycoforms and FCGR3A binding.

(A) Total agalactosylated (G0 class) glycans prevalence correlates positively with ADCVI activity. (B) Bigalactosylated (G2 class) glycans prevalence correlates negatively with NK cell ADCVI activity. (C) Bulk plasma antibody affinity for FCGR3A, measured as Biacore response units, correlates positively with ADCVI activity. (D) Impact of enzymatic treatment with a galactoside on agalactosylated glycan and (E) bigalactosylated glycan content. (F) Binding of treated and untreated antibody samples to FCGR3A beads. (A–C and F) Individual symbols represent each subject tested, and horizontal lines represent the median for a given group of clinically related subjects.