Table 5.
Comparison of GeXP assay and conventional single PCR for detecting seven aminoglycoside-resistance genes
| Resistance genes | GeXP assay |
Conventional single PCR |
Measures of agreement |
||
|---|---|---|---|---|---|
| Positive | Negative | Total | Kappa values* | ||
|
aac(3)-II |
Positive |
37 |
2 |
39 |
0.831 (P=0.000) |
| Negative |
2 |
15 |
17 |
||
| Total |
39 |
17 |
56 |
||
|
aac(6')-Ib |
Positive |
11 |
3 |
14 |
0.846 (P=0.000) |
| Negative |
0 |
42 |
42 |
||
| Total |
11 |
45 |
56 |
||
|
aac(6')-II |
Positive |
8 |
2 |
10 |
0.810 (P=0.000) |
| Negative |
1 |
45 |
46 |
||
| Total |
9 |
47 |
56 |
||
|
ant(3″)-I |
Positive |
40 |
2 |
42 |
0.909 (P=0.000) |
| Negative |
0 |
14 |
14 |
||
| Total |
40 |
16 |
56 |
||
|
aph(3')-VI |
Positive |
10 |
0 |
10 |
0.887 (P=0.000) |
| Negative |
2 |
44 |
46 |
||
| Total |
12 |
44 |
56 |
||
|
armA |
Positive |
40 |
2 |
42 |
0.810 (P=0.000) |
| Negative |
2 |
12 |
14 |
||
| Total |
42 |
14 |
56 |
||
| rmtB | Positive |
14 |
3 |
17 |
0.825 (P=0.000) |
| Negative |
1 |
38 |
39 |
||
| Total | 15 | 41 | 56 | ||
*As a rule of thumb, values of Kappa from 0.40 to 0.59 are considered moderate, 0.60 to 0.79 substantial, and 0.80 outstanding.