Table 4.
Primer pairs used for sequencing the mitogenome of Lophelia pertusa
| Forward primer | Reverse primer | Annealing T° | Fragment length | Reference |
|---|---|---|---|---|
| 5′-AAATCAAACGAGATTCCGAGAG-3′ |
5′-TCCATGGGGACTTCTCGTC-3′ |
53°C |
1198 bp |
this article |
| 5′-TCGACTGTTTACCAAAAACATAGC-3′ |
5′-AAYAACCTTCCATTGCATCC-3′ |
53°C |
1519 bp |
this article |
| 5′-TAGGAGTGGTTGGGAAATCG-3′ |
5′-CTTGGGGAAGCCAAATATGA-3′ |
53°C |
2563 bp |
this article |
| 5′-GAACAACAGGGGCAACAGAT-3′ |
5′-ATGGTGTCCCTGAAAAGTCG-3′ |
53°C |
2127 bp |
this article |
| 5′-GCAGACGCGGTGAAACTTA-3′ |
5′-TACCCCGGCTAAGACAACTG-3′ |
53°C |
2551 bp |
this article |
| 5′-TTGTGGGGCAAATCATTCTT-3′ |
5′-AATGAGAAAGCCCACAAGCA-3′ |
53°C |
1034 bp |
this article |
| 5′-CAACTCCGGTTTCTGCCTTA-3′ |
5′-TTTAAAAGAAAACTATGGAGGCCTAA-3′ |
53°C |
3060 bp |
this article |
| 5′-TTATTGGGCCTGTGTTTGGT-3′ |
5′-CCCACATATGAAAAGGAGCAAC-3′ |
53°C |
1604 bp |
this article |
| 5′-TGGGTGCTCTTTCTTCTGGT-3′ |
5′-AAATCCAATTGGTATATAATTTGTCA-3′ |
53°C |
1237 bp |
this article |
| 5′-ATCCCTCCTTTTGCAGGATT-3′ |
5′-CCCCAGAAGCTGTTGTGTTT-3′ |
53°C |
868 bp |
this article |
| 5′-GGCAATTGGTTCTGGGATAA-3′ |
5′-AAGCATACTAAAAGCCGTTCCA-3′ |
53°C |
1254 bp |
this article |
| 5′-GGTCAACAAATCATAAAGATATTGG-3′ |
5′-TAAACTTCAGGGTGACCAAAAAATCA-3′ |
45°C |
709 bp |
[58] |
| 5′-GCCGGTGCTATTACAATGCT-3′ | 5′-CAATCGATTCAAGCTCTTTTCA-3′ | 53°C | 1892 bp | this article |