Table 2.
MBCs of the extracts, compounds from Garcinia nobilis, Oricia suaveolens, Balsmocitrus camerunensis and reference drugs on documented strains and clinical MDR isolates
| Tested samplesa |
Microorganisms, strains and MBC (μg/ml)b |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
E. coli |
E. aerogenes |
K. pneumoniae |
P. stuartii |
P. aeruginosa |
M. tuberculosis |
||||||||||
| ATCC 10536 | AG 100 | AG 102 | ATCC 13048 | CM 64 | EA 27 | ATCC 11296 | KP55 | ATCC 29916 | NAE 16 | PA 01 | PA 124 | ATCC 27294 | MTCS1 | MTCS2 | |
|
Crude extracts |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| GNB |
256 |
512 |
>512 |
>512 |
>512 |
>512 |
256 |
>512 |
>512 |
- |
>512 |
- |
256 |
>512 |
512 |
| OSR |
256 |
>512 |
- |
>512 |
- |
- |
512 |
512 |
>512 |
512 |
- |
- |
>512 |
- |
>512 |
| OSW |
512 |
>512 |
- |
- |
- |
- |
256 |
512 |
- |
512 |
- |
- |
512 |
- |
>512 |
| BCB |
>512 |
- |
- |
512 |
- |
- |
>512 |
>512 |
- |
- |
- |
- |
- |
- |
- |
|
Compounds |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
1 |
>512 |
>512 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
2 |
128 |
256 |
256 |
256 |
>512 |
128 |
>512 |
>512 |
512 |
128 |
128 |
256 |
16 |
64 |
32 |
|
3 |
256 |
256 |
128 |
512 |
- |
512 |
- |
- |
- |
- |
- |
- |
128 |
- |
256 |
|
4 |
- |
128 |
256 |
- |
512 |
256 |
- |
- |
- |
- |
512 |
- |
>512 |
- |
>512 |
|
5 |
128 |
256 |
256 |
- |
- |
>512 |
>512 |
- |
>512 |
- |
128 |
256 |
64 |
128 |
128 |
|
8 |
>512 |
256 |
>512 |
- |
>512 |
256 |
>512 |
- |
- |
- |
- |
- |
256 |
>512 |
512 |
|
9 |
- |
256 |
>512 |
- |
512 |
>512 |
256 |
- |
- |
- |
- |
- |
- |
- |
- |
|
10 |
256 |
256 |
512 |
- |
- |
- |
- |
- |
>512 |
- |
- |
- |
512 |
- |
>512 |
|
11 |
>512 |
- |
- |
- |
- |
>512 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
13 |
>512 |
128 |
512 |
- |
>512 |
256 |
512 |
- |
- |
- |
256 |
>512 |
512 |
>512 |
>512 |
|
15 |
256 |
512 |
>512 |
- |
- |
512 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
16 |
256 |
256 |
- |
- |
|
>512 |
- |
- |
>512 |
- |
- |
- |
>512 |
- |
- |
|
17 |
- |
256 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
18 |
- |
512 |
- |
- |
- |
512 |
- |
- |
- |
512 |
- |
- |
- |
- |
- |
|
19 |
- |
512 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
20 |
- |
>512 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
|
21 |
>512 |
512 |
- |
- |
- |
512 |
512 |
- |
- |
- |
- |
- |
- |
- |
- |
|
22 |
>512 |
512 |
512 |
>512 |
256 |
- |
- |
>512 |
- |
- |
- |
- |
- |
- |
- |
| RA | <4 | 32 | >512 | 128 | - | 128 | 8 | 64 | - | 128 | 256 | 256 | 2 | 256 | 16 |
aTested samples [methanol-dichloromethane 1:1 extract of the stem bark of Garcinia nobilis (GNB), the wood (OSW)roots (OSR) of Oricia suaveolens, and stem bark of Balsmocitrus camerunensis (BCB); compounds isolated from the stem bark of Garcinia nobilis (1–5), Oricia suaveolens woods (6–11) and roots (12–17), and the stem back of Balsmocitrus camerunensis (18–22); caroxanthone (1), 4-prenyl-2-(3,7-dimethyl-2,6-octadienyl)-1,3,5,8-tetrahydroxyxanthone (2), smeathxanthone A (3), 8-hydroxycudraxanthone G (4), morusignin I (5), stigma-5-en-3-ol (6), 20(29)lupen-2-ol (7), evoxanthine (8), norevoxanthine (9), 1-hydoxy-2,3-dimethoxy-10-methylacridone (10), 1,3-dimethoxy-10-methylacridone (11), kokusaginine (13), 1-hydroxy-4-methoxy-10-methylacridone (15), syringaresinol (16), limonin (17), 1-hydroxy-3,6-dimethoxy-8-methylxanthone (18), xanthoxyletin (19), imperatorin (20), 6,8-dimethoxycoumarin (21), umbelliferone (22)]; RA: reference antibiotics were chloramphenicol for bacteria, isoniazid for M. tuberculosis; b(−): MBC not determined as the MIC was above 512 μg/ml.