Table 2.
Bacteria strains and plasmids used in this study
| Strains or plasmids | Characteristics | Source or reference |
|---|---|---|
|
Escherichia coli |
|
|
| DH5α |
Transformation recipient strain |
[47] |
| S17-1λ |
Conjugation donor strain |
[48] |
|
C. crescentus |
|
|
| NA1000 |
Also CB15N, synchronizable derivative of wild-type CB15 |
[49] |
| MM46 |
NA1000 ΔnczA (ΔCCNA_02473) |
This work |
| MM47 |
NA1000 ΔczrA (ΔCCNA_02809) |
This work |
| MM48 |
NA1000ΔczrAΔnczA |
This work |
| MM46+ |
MM46 xylX::nczA |
This work |
| MM47+ |
MM47 xylX::czrA |
This work |
|
Plasmids |
|
|
| pGEM-T Easy |
Cloning vector; Ampr |
Promega |
| pRKlacZ290 |
pRK2-derived vector with a promoterless lacZ gene; Tetr |
[50] |
| pNPTS138 |
Suicide vector used for gene disruption containing oriT and sacB; Kanr |
D. Alley |
| pNPT228XNE | xylX locus in pNPT228; Kanr | [51] |