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. 2013 Apr 15;12:129. doi: 10.1186/1475-2875-12-129

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Copyright © 2013 Kangwanrangsan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Localization of PyCPW-WPC-1 in sexual-stage parasites. Using an immunofluorescent assay (IFA), PyCPW-WPC-1 was found to be localized to the surface of developing ookinetes, including zygotes, retorts, and mature ookinetes (shown in green). The surface of developing ookinetes was stained with antibodies targeting Pys25 (red), a well-known ookinete surface protein. Nuclei were stained by DAPI (blue). The merged images represented the colocalization of PyCPW-WPC-1 and Pys25 on the parasite surface. Parasite morphology was analysed by differential interference contrast (DIC) imaging. The bar indicates 5 μm.