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. 2013 Apr 26;8(4):e62344. doi: 10.1371/journal.pone.0062344

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© 2013 Charlermroj et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The detection of (A) Acidovorax avenae subsp. citrulli (Aac) and (B) no antigen using eight antibodies-coated microsphere and R-Phycoerythrin (RPE) labeled antibodies, including 11E5 antibody. The detection of (C) Aac, (D) chilli vein-banding mottle virus (CVbMV), (E) watermelon silver mottle virus (WSMoV), (F) melon yellow spot virus (MYSV) detection and (G) no antigen with seven antibodies- coated microsphere and RPE-labeled antibodies without using 11E5 antibody. X-axis is antibody-coated microsphere and y-axis is median fluorescent intensity (MFI) from each RPE-labeled antibody. (H) Summary of selected antibody pair sets for the detection of the four plant pathogens.