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. 2013 Apr 26;8(4):e61482. doi: 10.1371/journal.pone.0061482

Figure 6. The PbCP1 2TMD region mediates trafficking to the discrete punctuate structures in the RBC cytosol.

Figure 6

(A) Alignment of PbCP1 and its paralogue Pb400 shows 77% identity. The signal peptide is highlighted in yellow, the PEXEL motif in purple and the predicted 2TMD region in red (Pb400) and blue (PbCP1), respectively. (B) Wild-type Pb400 is episomally expressed as a GFP fusion protein and localises to the RBC cytosol in live PbANKA parasites. Replacement of the predicted Pb400 2TMD and downstream region with that of PbCP1 led to the localisation of the resulting Pb400PbCP1TMDGFP chimera to the discrete extra-parasitic structures in the RBC cytosol. In contrast, replacement of the putative PbCP1 2TMD and downstream region with that of Pb400 abolished trafficking of PbCP1Pb400TMDGFP to the punctuate structures.