Figure 7. The TMD region of IBIS1 is not sufficient to mediate trafficking to the extra-parasitic structures.

The predicted PbCP1 and Pb400 2TMD and downstream region, respectively, were replaced with the single TMD (highlighted in brown in the schematic structure beside the panel) and downstream region of IBIS1 (17). The resulting fusion proteins PbCP1_IBIS1TMDGFP and Pb400_IBIS1TMDGFP, however, did not display the punctuate localisation pattern as seen in wild-type IBIS1-GFP and were only exported into the RBC cytosol.