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. 2013 Apr 26;8(4):e62509. doi: 10.1371/journal.pone.0062509

Figure 3. miR-132 and miR-212 are not critical for IFNβ induction in MEFs infected by Sendai virus.

Figure 3

MEFs were isolated from wild-type or MSK1/2 double knockout mice and stimulated for the indicated times with 400 ng/ml PMA. Total RNA was isolated and pri-miR-132/212 levels determined by qPCR as described in the methods (A). MEFs from wild-type or miR-132/212 knockout mice were infected with Sendai virus for the indicated times. Total RNA was isolated and the levels of IFNβ (B), pri-miR-132/212 (C) and nur77 (D) determined by qPCR. In each case fold stimulation was calculated relative to the unstimulated wild-type samples, and error bars represent the standard deviation of 4 independent cultures from 4 mice per genotype. For (C) and (D) *indicates a p value of <0.05 relative to the unstimulated control sample.