Figure 5. Identification of mouse testicular proteins binding to Dazap1 3′UTR.

(A) Diagram of Dazap1 3′UTR showing the locations of the various RNA probes used in B-D. (B) UV cross-linking of ³²P-labeled RNA probes from various regions of Dazap1 3′UTR and testicular proteins from mice at different ages. Asterisks point to proteins showing increasing expression during testis development. (C) UV cross-linking of probes from the C region and adult testis lysates. (D) RNA affinity pulldown assays of Dazap1 3′UTR fragments. Adult testis lysates were incubated with biotin-labeled RNAs and affinity purified by streptavidin-agarose beads. Bound samples were separated by 10% SDS-PAGE and proteins with specific binding were identified by LC/MS/MS spectrometry.