Table 4. Primer Sequences.
| A. RT-PCR primers for AKAP13-gene trap splicing | ||||
| Primer Name | Location (Mutant) | Sequence (5'-3') | Size (bp) | |
| MJS218 | Exon 8 (ΔBrx) | ACACCCAAGATGAAGCAAGG | 441 | |
| MJS219 | Exon Brx (ΔBrx) | AATTTCGGACCTGTGTGAGC | 573 | |
| MJS220 | Exon 21–22 (ΔGEF) | TGGAGTTGGCAATGATGAGA | 674 | |
| AKAPlbc-F1_MS | Exon 27 (ΔPKC) | TGAAGAGCACAACAGGAAGG | 432 | |
| MJS213 | Gene Trap (Univ. Rev) | TAATGGGATAGGTCACGT | ||
| B. Long-Range PCR primers in the gene trap construct | ||||
| Primer Name | Location | Sequence (5'-3') | ||
| MJS236 | βGal (Rev) | CCCTGCCATAAAGAAACTGTTACCC | ||
| MJS237 | Neo | GTGGAGAGGCTATTCGGCTATGACT | ||
| C. Genotyping primers | ||||
| Primer Name | Allele Identified | Sequence (5'-3') | Size (bp) | |
| MJS299 | Univ. ΔBrx (For) | TGGCATCTACCCAGGATCTC | ||
| MJS390 | WT ΔBrx (Rev) | CAAAGGCCATCTGCACACC | 1697 | |
| MJS284 | GT ΔBrx (Rev) | GTGAGGCCAAGTTTGTTTCC | 1275 | |
| MJS274 | Univ. ΔGEF (For) | TACCAAATAACAGTGCCTGCTCTCC | ||
| MJS253 | WT ΔGEF (Rev) | ATCTTGAGTGTGCGGATGTGATGTA | 1533 | |
| MJS214 | GT ΔGEF (Rev) | AGTATCGGCCTCAGGAAGATCG | 1182 | |
| MJS339 | WT ΔPKC (For) | TGTCTCTGGCCTGTTTGTGA | 1112 | |
| MJS340 | WT ΔPKC (Rev) | TCGGAAGAGGTTAAGGGACA | ||
| MJS272 | GT ΔPKC (For) | ACATTTCCCCGAAAAGTGC | 435 | |
| MJS260 | GT ΔPKC (Rev) | GGCTCACACTGGGTTCAATC | ||
(A) RT-PCR primers for verifying AKAP13 gene-trap splicing events are listed. The primer locations and mutant line verified are indicated. The size of the RT-PCR product is given in base pairs (bp). (B) The common long-range PCR primers within the gene-trap construct are listed. These primers were used with AKAP13 specific genomic DNA primers to identify the gene-trap insertion. (C) The genotyping primers used to identify the wild-type and mutant allele for the three mutant mouse lines are listed. The primer direction is also given: forward (For) and reverse (Rev). The size of the PCR product is given in base pairs (bp).