. 2013 Apr 26;8(4):e59242. doi: 10.1371/journal.pone.0059242

Table 1. Virulence-associated genes typical for E. coli isolated from diarrheic pigs (iVAGs) and isolated from extraintestinal disease (eVAGs) and phylogenetic origin of 104 porcine non-hemolytic E. coli isolates.

gene(s) or operon	description	prevalence in E. coli (n = 104) from healthy pigs of this study in%	prevalence in E. coli (n = 93) from wild boars of the study Römer et al. [17] and of our actual study (#) in%
iVAGs ¹
est-1a	Heat stabile enterotoxin 1	0	3.2
est-2	Heat stabile enterotoxin 2	2.9	3.2
fedA	Subunit of F18 fimbriae	0	1.1
stx2e	Shigatoxin 2e	1.9	5.4
fanA	Subunit of F5 fimbriae	0	0
fimF41a	Subunit of F41 fimbriae	0	0
fasA	Subunit of F6 fimbriae	1.0	0
eltB-Ip	Heat labile enterotoxin 1	0	0
faeG	Subunit of F4 fimbriae	0	0
eVAGs ²
Adhesins
aatA	APEC autotransporter	16.3^*	1.1 #^*
afa/draB	Afimbrial/Dr antigen-specific adhesin	1.0	0
csgA	Curli fibre-encoding gene	78.8	87.1
fimC	Type 1 fimbriae (D-mannose specific adhesin)	91.3^*	100^*
fimH	Type 1 fimbriae (D-mannose specific adhesin)	96.1^*	83.9 #^*
hra	Heat-resistant agglutinin	13.5^*	23.7^*
H7	H7 flagellar antigen	15.4	11.8 #
iha	Iron-regulated-gene-homologue adhesin	0^*	5.4^*
mat	Meningitis-associated and temperature-regulated fimbriae	81.7^*	48.4^*
papAH	Pilus associated with pyelonephritis	1.9	0
papC	Pilus associated with pyelonephritis	9.6^*	0^*
papEF	Pilus associated with pyelonephritis	4.8	0
papGII	Pilus associated with pyelonephritis	5.8	0
papGIII	Pilus associated with pyelonephritis	1.0	0
papGII/III	Pilus associated with pyelonephritis	0	0
sfa/focCD	S fimbriae (sialic acid-specific) and F1C fimbriae	4.8	5.4
tsh ¹	Temperature sensitive hemagglutinin	16.3^*	4.3^*
yqi	APEC fimbrial adhesin	27.9	30.1 #
Iron acquisition
chuA	Heme receptor gene (E. coli haem utilization)	19.2^*	39.8^*
feoB	Major bacterial ferrous iron transporter, iron(II) transport system	98.1^*	73.1 #^*
fyuA	Ferric Yersinia uptake (yersiniabactin receptor)	26.9	30.0
ireA	Iron-responsive element (putative catecholate siderophore receptor)	9.6	3.2
iroN ¹	Catecholate siderophore (salmochelin) receptor	21.2^*	10.8^*
irp2	Iron repressible protein (yersiniabactin synthesis)	22.1	19.4
iucD ¹	Aerobactin synthesis	26.0^*	1.1^*
sitD chr.	Salmonella iron transport system gene	1.0^*	11.8^*
sitD ep.¹	Salmonella iron transport system gene	14.4^*	5.4^*
iutA	Aerobactin receptor	36.5^*	5.4 #^*
Protectins/Serum resistance
cvi/cva ¹	Structural genes of colicin V operon (Microcin ColV)	19.2^*	6.5^*
neuC	K1 capsular polysaccharide	2.9	0
kpsMT II	Group II capsule antigens	21.2	24.7
ompA	Outer membrane protein	94.2^*	100^*
traT ¹	Transfer Protein	51.9	45.2
Toxins
astA	EAST1 (heat stable cytotoxin associated with enteroaggregative E. coli)	43.3	39.2 #
sat	Secreted autotransporter toxin	1.0	3.2
hlyA	Haemolysin A	3.8	0
cnf1/2	Cytotoxic necrotising factor 1/2	6.7	10.8
Invasins
gimB	Genetic island associated with neonatal meningitis	0	0
ibeA	Invasion of brain endothelium	3.8^*	16.1^*
tia	Toxigenic invasion locus in ETEC isolates	3.8	5.4
Miscellaneous
pic	Serine protease autotransporter	4.8	6.5
malX	Pathogenicity-associated island marker CFT073	7.7	16.1
ECoR group
A		63.5^*	29.0^*
B1		17.3^*	32.3^*
B2		4.8^*	21.5^*
D		14.4	17.2

Comparison to E. coli from wild boars of an already published study.

differences between both groups statistically significant with p<0.05

n.t. = not tested

iVAGs were included since it was shown that an iVAG can affect colonization [58].

eVAGs were included since it was shown that eVAGs correlated with successful intestinal E. coli colonization in pigs [29].