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Breeding design. Monogamous boxes represent offspring conceived in random mate-assignment cages and promiscuous boxes represent offspring conceived in promiscuously breeding enclosures. Direct competition between second-generation, cage-born promiscuous line and monogamous line mice was conducted in semi-natural enclosures. Female odor and mating preferences for third-generation, cage-born promiscuous line and monogamous line mice was conducted in mate-choice arenas.
