Figure 5. Effect of rapamycin on the viability of S. cerevisiae EC1118 cells during alcoholic fermentation.

The synthetic medium contains 142 mg/L assimilable nitrogen (SM142) and 5% lipid factors (LF5%), with or without 20 nmol/L rapamycin. Viability was measured by flow cytometry after propidium iodide staining. The graphs are the result of smoothing of measurements series (at least 3 repetitions) using the software R. Smoothing obtained is framed by a confidence interval calculated at 95%.