Figure 7. Effect of the nutrient imbalances on HSP12 stress gene expression in S. cerevisiae EC1118 during alcoholic fermentation.

The synthetic medium contained 71 mg/L (SM71) or 142 mg/L (SM142) assimilable nitrogen, and 5% or 100% lipid factors (LF5% or LF100%). Fluorescence of the GFP reporter protein, produced under the control of the HSP12 promoter, was measured by flow cytometry. The graphs are the result of smoothing of measurements series (at least 3 repetitions) using the software R. Smoothing obtained is framed by a confidence interval calculated at 95%.