Figure 1. Identification and characterization of proteins co-purifying with Nak1-TAP, Sog2-TAP and Cka1-TAP. (A) List of proteins identified by mass spectrometry co-purifying with S. pombe Nak1-TAP, Sog2-TAP and Cka1-TAP. Proteins were purified from cycling S. pombe cells expressing Nak1-TAP (JG15615), Sog2-TAP (JG16552) or Cka1-TAP (JG15429). Only proteins identified with at least two peptides are included. For a full list of identified proteins see the Table S1. Proteins found in other unrelated purifications (common contaminants) are omitted from this table. Number of unique peptides and sequence coverage are indicated. (B) Summary of phosphorylation sites identified by mass spectrometry. Protein purifications were performed as described in (A). Phosphorylation sites identified on Nak1, Cka1 and Sog2 in this study are indicated. New phosphorylation sites identified are indicated in bold. Sites with the R-X-X-S consensus motif are indicated in red. Asterisks indicate sites with the CK2 consensus motif S/T-X-X-D/E.