Table 1. Oligonucleotides used to construct chk1 alleles.
∆KA1: |
GTAAGAACAATCGGCTTCCCCTTTCAGGAGGACAAATTTC |
∆½KA1: |
GTAAGAACAATCGGCTTCCCTTTCCGTTTATCATGTAAAT |
∆492–496: |
GGGTAAAAGATGCGGCCGCCTGTAAGAACAATCGGCTTCC |
∆485–496: |
GGGTAAAAGATGCGGCCGCCTATTGAACTGACAACGTTCT |
∆485–491: |
CCGCCATTTTGTGAAACATCTATTGAACTGACAACGTTCT |
∆488–489: |
TTTTGTGAAACATCTGTAAGCGGCTTCCCTATTGAACTGA |
IV488–489AA: | TGTGAAACATCTGTAAGTGCTGCCGGCTTCCCTATTGAAC |