Figure 6. Depleting CDK8 enhances lipid accumulation in endometrial cancer AN3 CA cells. (A–D) Quantification of gene expression by using qRT-PCR. (E–F) The phase contrast images were acquired, showing lipid droplet accumulation in cells with depleted CDK8 (F) compared with the control (E). (G) Cells were stained with Nile Red and subjected to flow cytometry assays to quantify the lipid levels.