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. 2013 Jan 24;13:16. doi: 10.1186/1471-2180-13-16

Table 4.

Growth media used in this work

Medium Composition
RPMI
8.4 g L-1 RPMI 1640 (Sigma-Aldrich R1383), 2 g L-1 glucose, 0.165 M 3-(N-morpholino propanesulfonic acid (MOPS), adjusted to pH 7.3 with 10 N NaOH
YNB
6.7 g L-1 Yeast Nitrogene Base (Sigma Y1250), 2 g L-1 glucose, 0.165 M 3-(N-morpholino propanesulfonic acid (MOPS), adjusted to pH 7.3 with 10 N NaOH
YPD
Sufficient iron medium: Yeast extract (10 g L-1) peptone (20 g L-1) dextrose (20 g L-1) (Sigma-Aldrich Y1375)
RIM Restricted iron medium; YPD + 200 μM bathophenantroline disulfunate (BPS) (Sigma 146617)